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Part:BBa_K957011:Design

Designed by: Steven Tiwen Chen & Dylan Patrick Webster   Group: iGEM12_Cornell   (2012-10-28)


Salicylate inducible MtrB-polyHis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 576
    Illegal BamHI site found at 1073
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 408
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Constructed using non-BioBrick AscI cutsite of BBa_K957000.


Source

mtrB is originally derived from the mtrCAB operon of Shewanella oneidensis MR-1; BioBrick part BBa_K098994 was used as DNA source.

The salicylate sensitive region was isolated via PCR from a plasmid containing BBa_K228227, designed by PKU_Beijing 2009.

References